The Patterns of Labeling of Germinal-center Cells with Tritiated Deoxycytidine
نویسندگان
چکیده
Autoradiographic studies of lymphoid tissue with tritiated thymidine ([3H]TdR) have repeatedly demonstrated that thymus lymphocytes and germinal-center cells of lymph nodes and spleen are characterized by a weak labeling, whereas the majority of lymph node lymphocytes which occur outside of the germinal centers are intensely labeled (1-7) . An earlier study on mice by Osogoe and Ueki (8), on the other hand, has revealed that thymus lymphocytes are much more intensely labeled in vivo with tritiated deoxycytidine ([3H]CdR) than with [3 H]TdR, and that the intensity of [ 3H]TdR labeling of these cells is significantly decreased by the simultaneous administration of unlabeled CdR . These findings indicate that, in thymus lymphocytes, CdR which is circulating in a considerable amount (9) is utilized for the formation of DNAthymine and hence the incorporation of [3H]TdR into DNA-thymine in these cells is remarkably decreased . As for the synthesis of DNA-thymine in the germinal-center cells of lymph node and other lymphoid tissues, any evidence in support of such a utilization of CdR has not yet been obtained . The present study was therefore undertaken to examine the patterns of labeling of the germinal-center cells with [3H]CdR in comparison with those labeled with [ 3 H]TdR, and to gain further information on the utilization of CdR in lymphocytes by autoradiographic techniques.
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ورودعنوان ژورنال:
- The Journal of Cell Biology
دوره 57 شماره
صفحات -
تاریخ انتشار 1973